環(huán)境化學(xué), 2022, 41（6）: 2143-2145.
Abstract To establish a method for the simultaneous determination of diosmin, hesperidin, 5,7 dimethoxycoumarin, limonin and nomilin in Citri Sarcodactylis Fructus by high performance liquid chromatography coupled with diode array and charged aerosol detector (HPLC-DAD-CAD). Results: The concentrations of diosmin, hesperidin, 5,7-dimethoxycoumarin, limonin and normilin were 106.6—533 ng (r=0.9998), 102.4—512 ng (r=0.9999), 103.0—515 ng (r=0.9999) and 82.2—411 ng (r=0.9996), 127.6—638 ng (r=0.9994), respectively The average recoveries were 105.04%, 107.19%, 98.39%, 105.01% and 94.33%, respectively. The average recoveries were 105.04%, 107.19%, 98.39%, 105.01% and 94.33%, respectively. RSD values were 1.96%, 1.67%, 2.72%, 2.39% and 0.45%, respectively. The contents of hesperidin, diosmin, 5,7-dimethoxycoumarin, limonin and nomilin in 8 batches of samples were 0.026%—0.168%、0.036%—0.081%、0.064%—0.256%、 0.016%—0.084% and 0.068%—0.116%, respectively. Conclusion: The established HPLC-DAD CAD method is stable, reliable and reproducible, which can provide a reference for the study of quality control methods of Citri Sarcodactylis Fructus.
本研究中對照品：香葉木苷、橙皮苷、5,7-二甲氧基香豆素和檸檬苦素，均購自于四川恒誠致遠生物科技有限公司（Naturewill biotechnology Co., Ltd.）
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